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Carl Zeiss
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COMSOL Inc
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Noldus Information Technology
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Becton Dickinson
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MetaMorph Inc
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MetaMorph Inc
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Healthanywhere Inc
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MetaMorph Inc
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MetaMorph Inc
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CellTool Inc
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Noldus Information Technology
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Matec Instrument
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Image Search Results
Journal: European journal of immunology
Article Title: Guidelines for the use of flow cytometry and cell sorting in immunological studies
doi: 10.1002/eji.201646632
Figure Lengend Snippet: Examples for performance tracking with and without a CS&T module 130. (A) A Levey-Jennings chart of a weekly measured performance for one parameter (out of 10) is shown. The cross in red indicates a failure in the performance check (a higher PMT-Voltage is needed to reach the target values of the beads, which corresponds to a loss of sensitivity). After checking and changing the band-pass filter in front of the corresponding PMT, the performance is measured again and is compared to the previous situation (blue dots). With the correct band-pass filter installed, the performance of the PMT is back to the previous level. The graph is taken from a CS&T-Cytometer Performance Report of a BD FACSCanto II equipped with 3 lasers. (B) The histogram of channel A of the violet 405 nm laser shows the corresponding measurement to the situation described above in (a) and is taken from a self-defined, instrument-specific calibration worksheet. The blue population represents the “standard” setup (with a 510/50 band-pass filter in front of the PMT of channel A, where the beads are reaching the respective target values (brackets). The red curve shows a measurement with a 610/20 nm band-pass filter instead. The beads are clearly outside the target values and the positive and negative populations are barely separated from each other. This is an example, how one can easily track basic instrument performance without having a separate software module available.
Article Snippet: The Cytometer Setup and
Techniques: Cytometry, Software